山东医药2011，Vol.51Issue(42)：14-16,3.
转染CXCR4基因支气管上皮细胞向SDF-1的迁移观察
Observation on chemotaxis towards SDF-1 of bronchi epithelial cell transfected CXCR4 gene
摘要
Abstract
Objective To observe the directional migration towards chemokine SDF-1 of Human Bronchi Epithelial (HBE) cells transfected chemokine receptor-CXCR4. Methods The CXGR4 protein was detected in HBE cells by imtnu nofluorescence and flow cytometry. The CXCR4 lentiviral vector was conlmcted and transfected to HBE cells. The CXCR4 mRNA and protein in HBE cells transfected CXCR4 was delected by real-time quantitative PCR and Western blot. The HBE cells' directional migration towards SDF-1 through Transwell chamber experiment was observed. Results The protein expression level of normal HBE cells was low. Succeeded in constructing the lentiviral vector of CXCR4. The CXCR4 mRNA and protein in transfecled CXCR4 got a relative high level expression. The amount of cells directionally migrating to SDF-1 increased significantly compared to cells without SDF-1 added (all P <0.05. The fells reached to back membrane were 42.8 ±2.8,47.6 ±6.2,57.8 ±3. 3,62.4 ±5. 0,89. 8 ±4. 7,100. 0 ±4. 8 with final concentration of SDF-1 respec tively 1,3,10,30,100,300 nM) and this was concentration-dependent (P<0.05). Conclusion The directional migra tion towards chemokines SDF-1 of HBE cells transfected chemokine receptor-CXCR4 increased significantly.关键词
趋化因子受体/CXCR4基因/趋化因子/支气管上皮细胞/趋化作用/气管组织工程技术Key words
chemokine receptor/ CXCR4 gene/ chemokine/ bronchi epithelial cell/ chemotxis/ tracheal tissue engineering technology分类
生物科学引用本文复制引用
刘军,韩云,石文君..转染CXCR4基因支气管上皮细胞向SDF-1的迁移观察[J].山东医药,2011,51(42):14-16,3.基金项目
辽宁省教育厅高等学校科研基金资助项目(J2010636). （J2010636）
