山东医药2011，Vol.51Issue(42)：24-26,3.
无精子症患者的染色体核型、Y染色体微缺失分析及诊断
Analysis and diagnosis on chromosomes karyotype and AZF microdeletion in patients with azoospermia
摘要
Abstract
Objective To observe the frequency of karyotype and AZF microdeletion on Y chromosome in azoospermia patients, and to explore the diagnostic methods. Methods G display-zone technique, Multi-PCR technology and ELISA method were used to test chromosomes karyotype. AZF genes and sex hormones in 96 azoospermia and 40 controls, respec tively. TESA/PESA were displayed to determine obstructive azoospermia. Results There were 27 cases with chromosome disorder in azoospermia patients and 2 cases in 40 controls. 7 cases with AZF microdeletion were found in azoospermia pa tients , include 5 AZFc, 1 case AZFb + c and 1 case AZFa + b + c, none in controls. PESA/TESA were performed in 42 cases in azoospermia patients, 26 cases were diagnosed to be obstructive azoospermia( OA) , others were non-obstructive azoospermia{ NOA) , the levels of FSH and LH in OA were higher than those in NOA groupf P < 0. 01) , while T was lower (P <0.01). Conclusions Chromosome abnormalities and AZF microdeletion play an important role in azoospermia pa tients, genetics and semen hormone tests will provide important reference for treatment.关键词
无精子症/精子/不育/染色体核型/Y染色体/AZF微缺失/性激素Key words
azoospermia/ sperm/ infertility/ chromosomes kayotype/ Y chromosome/ AZF microdeletion/ sex hormone分类
医药卫生引用本文复制引用
刘春莲,景万红,彭亮,焦海燕,徐仙..无精子症患者的染色体核型、Y染色体微缺失分析及诊断[J].山东医药,2011,51(42):24-26,3.基金项目
宁夏医科大学面上科研基金资助项目(XM201040). （XM201040）
