大连工业大学学报2011，Vol.30Issue(5)：318-321,4.
酿酒酵母胞内代谢关键酶对乙醇的耐受性
Tolerance of key metabolic enzymes on ethanol in the vivo cell of Saccharomyces cerevisiae
摘要
Abstract
The activity of Glucose-6-phosphate dehydrogenase, pyruvate kinase, isocitrate dehydrogenase, malate dehydrogenase, ethanol dehydrogenase and isocitrate lyase in the EMP pathway and TCA cycle were detected in the medium with 2 %, 4%, 6 %, 8 % ethanol to analyze the tolerance of key metabolic enzymes on ethanol in the vivo cell of Saccharomyces cerevisiae. The results show that glucose-6-phosphate dehydrogenase activity was increased by 23. 65% when adding 8% ethanol; the activity of pyruvate kinase and alcohol dehydrogenase was decreased by 77. 7% and 62.72% when adding 2% ethanol, and the activity of isocitrate dehydrogenase is only 0.003 U; malate dehydrogenase and isocitrate lyase activities were increased by 256.25% and 276.16% when adding 8% concentration of ethanol.关键词
酿酒酵母/酶活力/乙醇/耐受性Key words
Saccharomyces cerevisiae/enzyme activity/alcohol/tolerance分类
轻工纺织引用本文复制引用
聂好,董亮,侯德文,李明达,王晓丹,赵长新..酿酒酵母胞内代谢关键酶对乙醇的耐受性[J].大连工业大学学报,2011,30(5):318-321,4.基金项目
“十一五”国家科技支撑计划项目(2007BAK36B01). （2007BAK36B01）
