大连工业大学学报2011，Vol.30Issue(5)：346-349,4.
人溶菌酶基因在毕赤酵母中的表达及其抗菌活性检测
Expression of the human lysozyme in Pichia pastoris and antibacterial activities test
摘要
Abstract
The HLY coding gene was cloned into P. Pastoris expression vector Ppic9k. The Ppic9k/HLY was verified by two enzyme digestion and sequencing and then transferred into P. Pastoris GS115 strain by electroporation. The recombinant strains were isolated by G418. The results of SDS-PAGE demonstrated that the fermentation supernatant contained the recombinant protein up to 32% of total proteins and its molecular weight is 15 ku. In vitro the recombinant protein has certain antimicrobial activity against M. Lysodeikticus, E. Coli, S. Aureas and B. Subtilis, which show that the successful expression of the recombinant HLY in P. Pastoris and thus provide the basis for its large-scale production using P. Pastoris system.关键词
人溶菌酶/毕赤酵母/pPIC9K/表达Key words
human lysozyme/Pichia pastoris/Ppic9k/expression分类
化学化工引用本文复制引用
夏清风,侯英敏,曹芳,金朝霞..人溶菌酶基因在毕赤酵母中的表达及其抗菌活性检测[J].大连工业大学学报,2011,30(5):346-349,4.基金项目
大连市优秀青年人才基金项目(2009J22DW036). （2009J22DW036）
