江西农业大学学报2011，Vol.33Issue(4)：665-669,5.
换锦花种胚和叶片的组织培养研究
Tissue Culture of Leaves and Embryo in Lycoris sprengeri
摘要
Abstract
Lycoris sprengeri Comes ex baker is one of the most beautiful bulbs but hasn' t been used in landscaping widely owing to lack of rapid propagation technique. The leaf blades and embryo of L. Sprengeri were cultured in vitro on MS mediums supplemented with different concentrations of plant growth regulator. The results showed that high induction rates, 75. 8% on average, were obtained in embryo culture. The most ideal callus induction medium for embryo culture was MS + 6 - BA 2 mg/L + 2,4 - D 2 mg/L + Sucrose 60 g/ L, while the most ideal clustered shoots induction medium was MS + 6 - BA 2 mg/L + 2,4 - D 1 mg/L + Su crose 30 g/L. The results of leaf tissue culture showed that the induction rate of young leaves was 65. 8% on average which was higher than that of mature leaves( 13. 5% on average). The most ideal induction medium for young leaves tissue culture was MS + 6 - BA 20 mg/L + NAA 1 mg/L + Sucrose 30 g/L. The results of growth regulator contrast test showed that higher NAA concentration was good for induction of roots, while higher 6 - BA concentration was good for induction of shoots.关键词
换锦花/石蒜属/组织培养/种胚/叶片Key words
L. Sprengeri/ Lycoris Herb. / tissue culture/ embryo/ leaves分类
农业科技引用本文复制引用
时剑,童再康,刘志高,高燕会,姜小凤,黄华宏..换锦花种胚和叶片的组织培养研究[J].江西农业大学学报,2011,33(4):665-669,5.基金项目
浙江省科技项目(2006C12003):浙江省创新团队项目(2009R50034)和杭州市科技项目(20080632B29) （2006C12003）
