江西农业大学学报2011，Vol.33Issue(4)：769-773,5.
副猪嗜血杆菌江西株的分离鉴定及生物学特性研究
Isolation, Identification and Chariterization in Biology of Haemophilus parasuis in Jiangxi Province
摘要
Abstract
In order to investigate the current situation of Haemophilus parasuis(Hps) infections in Jiangxi Province,and then provide a foundation for prevention and cure of disease caused by Hps. 20 strains of Gram negative, small polymorphic bacillus were isolated from 80 samples characterized by serofibrinous, from 32 large-scale farms in Jiangxi Province. The results of morphological and biochemisty tests and PCR all validated that these bacteria were Haemophilus parasuis. A comparison of the 16S rRNA sequence between Jiangxi iso lates and data posted on NCBI showed that the homology was about 97%. The study on its biological charac teristics showed than it requied nicotinamide adenine dinucleotide and serum of newborn calves added into the medium. The vitro-living time of this bacterium depended on the culture medium in which it was planted.关键词
副猪嗜血杆菌/生化鉴定/PCR鉴定/生物学特性Key words
Haemophilus parasuis/ biochemisty test/ PCR/ chariterization in biology分类
农业科技引用本文复制引用
宋德平,肖根辉,王萍,何后军,邬向东,黄冬艳..副猪嗜血杆菌江西株的分离鉴定及生物学特性研究[J].江西农业大学学报,2011,33(4):769-773,5.基金项目
江西省科技厅科技支撑计划(2009BNA0700)和江西省教育厅研究生创新基金(YC10A060) （2009BNA0700）
