福建农林大学学报（自然科学版）2011，Vol.40Issue(5)：494-500,7.
龙眼胚性愈伤组织LEC1基因cDNA克隆以及在体胚发生过程中的表达分析
Cloning of LEC1 gene from embryogenic callus and its expression analysis during somatic embryogenesis in longan
摘要
Abstract
LECl gene was cloned from embryogenic callus and its expression patterns were determined during somatic embryogenesis in longan ( Dimocarpus longan Lour. ). The RT-PCR (reverse transcription polymerase chain reaction) , RACE ( rapid amplification of cDNA ends) and TAIL-PCR were used to isolate the complete cDNA sequence of LECl from longan embryogenic callus, and the bioinformatics methods were used to analyze the cDNA sequence and putative amino acid sequence. The full length LECl cDNA, which had been submitted to the DDBJ/EMBL/CenBank database (the accession number was GU584089.2) , was 803 bp, in addition, the putative protein had 222 amino acids, and the identity to the other polypeptides varied between 35% -70%. Finally, the qRT-PCR (real-time quantitative transcription PCR) method was used to determine the mRNA transcription levels of the gene. LECl was expressed at the different stages during somatic embryogenesis, showing a single peak curve with the relative higher mRNA transcription level of LEC1 gene at the heart embryo stage and the torpedo embryo stage.关键词
龙眼/体胚发生/LEC1/基因克隆/序列分析/实时荧光定量PCRKey words
Dimocarpus longan/somatic embryogenesis/Leafy Cotyledon 1 /gene cloning/sequence analysis/real-time quantitative transcription PCR分类
农业科技引用本文复制引用
蔡英卿,赖钟雄,陈义挺,林玉玲,李惠华,张妙霞..龙眼胚性愈伤组织LEC1基因cDNA克隆以及在体胚发生过程中的表达分析[J].福建农林大学学报（自然科学版）,2011,40(5):494-500,7.基金项目
福建省重大科技平台建设(2008N2001),国家自然科学基金(31011787、30471204)资助项目. （2008N2001）
