福建农林大学学报（自然科学版）2011，Vol.40Issue(5)：501-510,10.
猪伪狂犬病病毒FZ株的分离鉴定及gD基因序列分析
Isolation and identification of porcine pseudorabies virus FZ strain and sequence analysis of the gD gene of the strain
摘要
Abstract
A wild-type pseudorabies virus (PRV) was isolated from an affected porcine in Fuzhou. Supematants from cell extracts of nose cavity mucus, brain, spleen, kindy, lymph were inoculated to the Vero cells and MDCK cells and significant cytopathogenic effect was produced. The isolated virus was confirmed by serological tests, PRV-Fluorescence antibody tests, experimental infection of animals and PCR tests. The virus isolate was designated as PRV-FZ. A pair of oligonucleotide primers were designed according to the sequences published by the GenBank in order to amplifiy gD gene of the PRV-FZ. A PCR product of approximately 1579 bp was amplified from PRV-FZ strain. The PCR product was inserted into PCAGGS vector and sequenced. Nucleotide sequencing revealed that this fragment contained an open reading frame of 1203 bp encoding a 400 aa protein. The gene was sequenced and compared with those of five other PRV strain in GenBank. The results showed that the gD gene of PRV-FZ shared 99.1% - 99.8% nucleotide homology and 98.5% -99.5% amino acid homology with strains. Meanwhile there are two notable transmembrane region in PRV-FZ gD by Bioedit and TMPRED; Structure prediction shows that there are two CAMP_PHOSPHO_SITE, five PKC_PHOSPHO_SITE, one CK2_PHOSPHO_SITE and three pires of disulfind.关键词
猪伪狂犬病毒/分离鉴定/FZ株/gD基因/序列分析Key words
pseudorabies virus/isolation and identification/FZ strain/gD gene/sequence analysis分类
农业科技引用本文复制引用
曾显成,吴异健,陈家祥,姚金水,吴宝成..猪伪狂犬病病毒FZ株的分离鉴定及gD基因序列分析[J].福建农林大学学报（自然科学版）,2011,40(5):501-510,10.基金项目
福建农林大学青年教师基金资助项目(06A13). （06A13）
