福建农林大学学报（自然科学版）2011，Vol.40Issue(5)：516-520,5.
苏云金芽胞杆菌BRC-HZP7杀虫晶体蛋白多样性分析
ICPs polymorphism of Bacillus thuringiensis BRC-HZP7
摘要
Abstract
Bacillus thuringiensis strain BRC-HZP7 was isolated from the soil of Wuyi mountain, which exhibited the high activity a-gainst Plutella xylostella larvae and Spodoptera exigua larvae. Biochemical reactions were tested for its biological characters. The cry gene types and their encoded proteins of BRC-HZP7 were identified by the methods of PCR-RFLP, SDS-PAGE and PMF. The results showed that cry1Aa, crylBa, cry1Ia, cry2Ab, cry2Ac and cry2Ae existed in BRC-HZP7. Eight interesting protein bands were picked out to analyze further by MALDI-TOF/MS. PMF matching analysis results showed that band 3,6,7,8 were ICPs. The band 6,7,8 were identified as Cry2Aa, Cry1Aa, Cry1Ba, respectively. The band 3 was possible partial fragment of Cry1Ba. The strain with abundant ICPs would be a good resource in pest biocontrol.关键词
苏云金芽胞杆菌/PCR-RFLP/cry基因/杀虫晶体蛋白/肽段指纹图谱Key words
Bacillus thuringiensis/PCR-RFLP/cry gene/insecticidal crystal proteins/peptide mass footprint分类
农业科技引用本文复制引用
沙莉,章淘,黄张敏,黄志鹏,关雄..苏云金芽胞杆菌BRC-HZP7杀虫晶体蛋白多样性分析[J].福建农林大学学报（自然科学版）,2011,40(5):516-520,5.基金项目
教育部博士点基金资助项目(20093515110010) （20093515110010）
福建省教育厅资助项目(JK2009012、JA10100) （JK2009012、JA10100）
福建省高校服务海西建设重点资助项目(闽教高(2009)8号,06085B05J) （闽教高(2009）
福建省自然科学基金资助项目(2007J0324). （2007J0324）
