四川医学2011，Vol.32Issue(11)：1679-1682,4.
极化心脏停搏液对成熟心肌细胞保护作用的研究
Protective effects of cardioplegia with tetrodotoxin on mature cardiomyocytes
杨朝坤 1杨双强2
作者信息
- 1. 宜宾市第二人民医院胸心外科,四川宜宾644000
- 2. 重庆医科大学附属第一医院胸心外科,重庆416000
- 折叠
摘要
Abstract
Objective To investigate the Effects of polarizing cardioplegia solution with sodium channel inhibitor tetrodo-toxin('lTX) through observating the intracellular free Na * concentration( [Na+]I) and Ca2+ concentration( [Ca2+]I)in isolated cardiomyocytes of mature rat. Methods Mature ventricular myocytes with beating were isolated from adult rat hearts by enzymatic dissociation, randomly created in Group Base,Group STH2(contrast group of ischemia/reperfusion) and Group TTX(treated group). Both Group STIC and Group TTX were arrested by St. Thomas No. 2 cardioplegia solution and TTX cardioplegia solution respectively, the arrest/re-beating cell model imitating MIRI being established, and imaged by laser scanning confocal microscopy ( LSCM) for measuring [ Na+ ]; and [ Ca2+ ] -, of cardiomyocytes in different period. Results [ Na+ ] , and [ Ca2 + ] -, of mature cardiomyocytes in both Group TTX and Group STH2 after re-beating was higher than that in Group Base ( P＜0. 01) , [ Na * ] I and [Ca2 + ]I in Group TTX was lower than that in Group STH2(P＜0. 01). Conclusion Contrast depolarized cardioplegia solution , polarized cardioplegia solution could alleviate ischemia reperfusion injury and intracellular Na+ and Ca2+ overload of mature cardiomyocytes.关键词
心脏停搏液/极化停搏/河豚毒素/钠通道/成熟心肌细胞/Na+/Ca2+Key words
cardioplegia/polarized arrest/tetrodotoxin/ sodium channel/mature cardiomyocytes/ sodium ion/Calciumion分类
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杨朝坤,杨双强..极化心脏停搏液对成熟心肌细胞保护作用的研究[J].四川医学,2011,32(11):1679-1682,4.
