分析化学2011，Vol.39Issue(10)：1567-1571,5.DOI:10.3724/SP.J.1096.2011.01567
四氨基酞菁锌负载二氧化硅纳米粒子的合成及其对细胞毒性的研究
Preparation of Tetraamino-Phthalocyanine Zinc Loaded Silica Nanoparticles and Its Cytotoxicity Study
摘要
Abstract
Positive charged silica nanoparticles, entrapping hydrophobic photosensitizer 2,9,16,23-tetraamino-phthalocyanine zinc (ZnPc(NH2 )4), have been synthesized in the nonpolar core of micelles by hydrolysis of triethoxyvinylsilane (TEVS) and 3-aminopropyltriethoxysilane (APTES). The water-soluble, stability, surface morphologies and charge were characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS) and UV-visible spectrophotometer. The as-prepared nanoparticles are highly monodispersed spheres with uniform diameter (about 20 nm), and stable in aqueous system. Its average ζ potential value is 28. 8 ± 2. 79 mV and exhibits strong absorption peak at 714 nm. In addition, encapsulation of ZnPc(NH2)4 in the silica nanoparticles prevented ZnPc ( NH2 )4 from leaking and enhanced the anti-photobleaching. The ZnPc ( NH2 )4 entrapped-nanoparticles (SiP2@ZnPc(NH2 )4) can efficiently generat singlet oxygen as measured by chemical probe 1,3-diphenylisobenzofuran (DPBF). The toxicity of silica nanoparticles to cells has been investigated by the incubation of the ZnPc(NH2)4 entrapped-nanoparticles and the nanoparticles without ZnPc(NH2)4(SiO2-NH2) with living cancer cells (HeLa, U251, PC-12). The experimental result reveals that both SiO2@ZnPc(NH2 )4 and SiO2-NH2 particles have no significant toxicity when the concentration of the particles is below 300 mg/L.关键词
二氧化硅纳米粒子/四氨基酞菁锌/微乳液/细胞毒性Key words
Silica nanoparticles/ Tetraamino-phthalocyanine zinc/ Microemulsion/ Cytotoxicity引用本文复制引用
庄欠粉,王金娥,朱志军,李峰,王振新..四氨基酞菁锌负载二氧化硅纳米粒子的合成及其对细胞毒性的研究[J].分析化学,2011,39(10):1567-1571,5.基金项目
本文系国家自然科学基金(No.21075118)和中国科学院项目(No.KJCX2-YW-H11)资助 （No.21075118）
