分析化学2011，Vol.39Issue(10)：1572-1576,5.DOI:10.3724/Sp.J.1096.2011.01572
高效液相色谱法检测食品接触产品中的三聚氰胺及其衍生物的迁移量
Determination of Melamine and Related Derivatives by High Performance Liquid Chromatography
摘要
Abstract
A novel High-performance liquid chromatographic method of determining monomer migration of melamine (MEL) and its derivatives including ammeline (ANE), ammelide (ADE) and cyanu-ric acid (CYA) was developed. Samples were immersed in simulants (H2O, 4% acetic acid, 10% ethanol), and then kept in incubator at 70 ℃ in a duration of 4 h. The immersed solutions were injected into HPLC column before the penetration through a 0. 45-μm microporous membrane. The separation was performed on a Phenomenex Luna NH2 column at 30 ℃ and the mobile phase of acetoni-trile (5 mmol/L) with PBS (pH =6. 5)(75:25, V/V) of isocratic elution was pumped at a flow rate of 1. 0 mL/min with DAD detection at 205 and 214 nm. Under the optimum conditions, the calibration curves were linear from 0. 5 to 10 mg/L with LODs (S/N = 3) of 0. 06, 0. 08, 0. 12 and 0. 15 mg/L for MEL, ANE, ADE and CYA, respectively. The method provided recoveries of 96. 4% - 101. 6% in the spiked concentration of 1. 0 mg/L, and the RSD of the peak height was between 2. 8% and 4. 4%. The satisfied experimental results indicated that the proposed method can be directly used for the determination of monomer migration of melamine and its derivatives in food contact material conveniently, rapidly and accurately.关键词
三聚氰胺/衍生物/食品接触产品/高效液相色谱/迁移Key words
Melamine/ Derivatives/ Food contact material/ High performance liquid chromatography/ Migration test引用本文复制引用
陈旻实,井伟,吕水源,李小晶,吴洪成,林柏玲,郑思远,陈锦权..高效液相色谱法检测食品接触产品中的三聚氰胺及其衍生物的迁移量[J].分析化学,2011,39(10):1572-1576,5.基金项目
本文系福建省科技计划重点项目(No.2009Y0001),国家质量监督检验检疫总局2011年度科技计划项目(No.20111K028),福建出入境 检验检疫局科技计划项目(No.FK2010-22)资助 （No.2009Y0001）
