分析化学2011，Vol.39Issue(10)：1589-1594,6.DOI:10.3724/SP.J.1096.2011.01589
基于芯片介电电泳的细胞特性分析及种类判断
Cell Characters Analysis and Type Identification on Dielectrophoresis Microchip
吴永杰 1徐溢 2彭金兰 3曹强 1曾萍2
作者信息
- 1. 重庆大学化学化工学院,重庆400030
- 2. 新型微纳器件与系统技术国家重点学科实验室,重庆400030
- 3. 微纳系统及新材料技术国际研发中心,重庆400030
- 折叠
摘要
Abstract
On the basis of the principle and technology of microfluidic chip dielectrophoresis (DEP), crossover frequencies of red blood cell (RBC), white blood cell (WBC) and HepG2 were tested on a homemade parabolic electrode dielectrophoresis microfluidic chip when the conductivity of the buffers was in the range of 200-1000 μS/cm and the applied excitation voltage at 5 V. Five parallel experiments were carried out and the relative error was less than 5%. Then, membrane conductance (Cmem) and membrane capacitance (Gmem) were calculated and analyzed. The result showed that electrical parameters of different types of cells and different levels of activity of cells were of great difference. In this study, the cell types and its biological activity was studied by using the cell DEP crossover frequency testing method.关键词
介电电泳/临界频率/膜电容/膜电导Key words
Dielectrophoresis/ Crossover frequency/ Membrane conductance/ Membrane capacitance引用本文复制引用
吴永杰,徐溢,彭金兰,曹强,曾萍..基于芯片介电电泳的细胞特性分析及种类判断[J].分析化学,2011,39(10):1589-1594,6.
