棉花学报2011，Vol.23Issue(5)：414-421,8.
棉花早熟芽黄突变体叶绿素荧光动力学特性研究
Research of Chlorophyll Fluorescence Dynamic Characteristic in A Cotton Virescent Mutant
摘要
Abstract
In this research, CCRI 58 and its space virescent mutant vsp were used as materials to analyze the change of primary photochemical of different position leaves(the first to the fifth leaves from the top) by chlorophyll a fluorescence transient measurement and the relevantly analytical method, HP-test on sunny days. The results showed that the maximum photochemical efficiency of photosystem Ⅱ Fv/Fm and performance index (Piabs) displayed a tendency of progressive increase in the wild type from the first leaf from the top to the fifth leaf from the top, while in the mutant, the young leaves contained less chlorophyll, and the value of Fv/Fm and Piabs in the second leaf from the top showed the lowest level, then increased to normal level 10 days later(the fifth leaf from the top). Compared to the wild type, the high relative variable fluorescence intensity of K-step (Wv) in the young leaves meant that the oxygen-evolving complex (OEC) got damaged, and the increasing of approximated initial slope of the fluorescence transient (At), the reduction of normalized total complementary area above the O-J-I-P transien(Sm), quantum yield for electron transport(φEo), probability that a trapped exciton moves an electron into the electron transport chain beyond QA-(ψo) revealed that the QA- accumulation was excessive in the acceptor side of PS Ⅱ, the electron transport chain got blocked. The changes in the parameters of absorption flux per reaction center (ABS/RC), trapped energy flux per reaction center (Tro/RC), electron transport flux per reaction center (Eto/RC), dissipated energy flux per reaction center (DIO/RC) showed that, in the early development of the leaf, the energy absorbed by reaction center was dissipated through heat and fluorescence. Above all, we speculated that because of the block of the chlorophyll biosynthesis at the early development of the young true leaves, the PS Ⅱgot damaged and showed lower photochemical reaction efficiency.关键词
棉花/芽黄突变体/叶绿素荧光/光系统ⅡKey words
cotton/ virescent mutant/ chlorophyll fluorescence/ PS Ⅱ分类
农业科技引用本文复制引用
田明爽,宋美珍,范术丽,庞朝友,喻树迅..棉花早熟芽黄突变体叶绿素荧光动力学特性研究[J].棉花学报,2011,23(5):414-421,8.基金项目
国家十一五“863”计划(2007AA100103),国家科技支撑计划(2008BAD97B05) （2007AA100103）
