棉花学报2011，Vol.23Issue(5)：427-432,6.
棉花种子特异性启动子的克隆及表达载体构建
Cloning Seed-specific Promoter from Cotton and Construction of Plant Expression Vector
摘要
Abstract
In order to study the expression of late embryogenesis abundant gene in cotton seeds, the 1262 bp 5'flanking sequence of D113 gene and the 1450 bp 5'flanking sequence of D34 gene were cloned by PCR from Gossypium arboreutn L. The similari-ty of cloned D113 gene promoter and D34 gene promoter compared with the sequence of Lea protein gene family which had been reported earlier were 97.43% and 94.27% , respectively. Two new plant expression vectors named Pbi121-D113 and Pbi121-D34 in which the reporter gene GUS is drived by Dl13 and D34 gene promoter were constructed after cutting two vec-tors Pgemt-D and Pbi121-T with two restriction enzymes HindⅢand xbal, subsequently recovered the small fragment from Pgemt-D recombined vector and the long fragment from Pbi121-T plant expression vector, and then ligation, transformation and identification were conducted. Finally, foundation has been set up for further research work in expression and function of this promoter.关键词
Lea蛋白:D113基因启动子/D34基因启动子/启动子分析/表达载体Key words
Lea protein/ Dl13 gene promoter/ D34 gene promoter/ promoter analysis/ plant expression vector分类
农业科技引用本文复制引用
琦明玉,陆才瑞,邹长松,王巧莲,宋国立..棉花种子特异性启动子的克隆及表达载体构建[J].棉花学报,2011,23(5):427-432,6.基金项目
转基因生物新品种培育重大专项(2009ZX08005-025B) （2009ZX08005-025B）
