棉花学报2011，Vol.23Issue(5)：454-459,6.
源库调节对Bt棉棉铃外源杀虫蛋白表达量影响的研究
Effect of Source-sink Regulation on the Content of Bt Endotoxin in Bt Cotton Boll
摘要
Abstract
The objective of this study was to investigate the change of insecticidal protein content under source-sink regulation in Bt cotton boll. The experiments were conducted at Yangzhou University farm during cotton growth season from 2005 to 2006, three cultivars with different boll sizes of Bt cotton, Sikang 1 (small boll), Sikang 3(middle boll), Kemian 6(big boll), were select-ed to study Bt endotoxin characteristics under source-sink regulation. The three cultivars were designed to be cut half leaves of the whole plant in 2005, and to be thinned half buds of the whole plant in 2006. The results showed that, with being cut half leaves of the whole plant, the boll volume, boll weight and seed weight decreased, and its effects on the boll volume and weight were greater in the big boll cultivar, reducing by 15.4% and 20.5% at 28 days after anthesis, respectively; in contrast, its effect on the seed weight was the biggest in the small boll cultivar, decreasing by 20.6% at 28 days after flowering. The Bt protein con-tents increased in the boll wall and seed, which increased by 23.5%~32.3%, 3.3%~18.8% at 21 days after anthesis, respectively. With half buds of the whole plant thinned, the boll volume, boll weight and seed weight were boosted, and the effects were the greatest in the big boll cultivar, increasing by 25.8%, 10.4%, 12.7% at 28 days after anthesis, respectively; in contrast, the Bt protein contents in the boll wall and seed decreased, the effects was greatest in the big boll cultivar too, reducing by 40.6%, 33.7% at 21 days after anthesis, respectively. There were significant negative correlations between the boll volume, boll weight and Bt endotoxin contents in the boll wall and seed under source-sink regulation, respectively, so was between seed weight and Bt endotoxin contents in the seed. The results suggest that the breeding and culture practice should aim at coordinating the rela-tion between the boll development and 3t endotoxin expression.关键词
Bt棉/源库调节/Bt蛋白/铃体积/铃重Key words
Bt cotton/ source-sink / emulation/ Bt protein/ boll volume/ boll weight分类
农业科技引用本文复制引用
吉荣龙,崔必波,黄志勇,费月跃,顾克余,孙扣忠,陈德华..源库调节对Bt棉棉铃外源杀虫蛋白表达量影响的研究[J].棉花学报,2011,23(5):454-459,6.基金项目
国家自然科学基金(30971727) （30971727）
江苏省重点实验室重大项目(10KJA210057) （10KJA210057）
江苏省青蓝工程和扬州大学新世纪学术带头人基金:江苏省自然科学基金企业博士计划(BK2009653) （BK2009653）
农业部国家转基因生物新品种培育重大专项(2009ZX08013-014B) （2009ZX08013-014B）
农业部国家转基因生物新品种培育重大专项(2009ZX08013-011B) （2009ZX08013-011B）
