环境与职业医学2011，Vol.28Issue(11)：684-686,3.
硫酸铟对V79细胞内活性氧和线粒体膜电位的影响
Effects of Indium Sulfate on Intracellular Reactive Oxygen Species and Mitochondrial Membrane Potential in V79 Cells
赵静珺 1肖卫2
作者信息
- 1. 陕西省疾病预防控制中心,陕西西安 710054
- 2. 苏州大学,江苏苏州 215123
- 折叠
摘要
Abstract
[ Objective ] To observe the changes of intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (A ψm) in Chinese hamster V79 cells induced by indium sulfate in vitro. [ Methods ] The cultured V79 cells were exposed to indium sulfate at various dosages (0.5, 1.0, 2.0, 4.0 and 8.0 mmol/L). The cell survival rate was checked with methyl thiazolyl tetrazolium (MTT). The changes of intracellular ROS and Aψm in V79 cells were analyzed by flow cytometry with 2', 7'-dichlorodihydrofluorescin diacetate (DCFH-DA) and 5, 5', 6, 6'-tetrachloro -1, 1', 3, 3'-tetraethyl-imidacarbocyanine iodide (JG-1) probe after being interfered for 12h. [ Results ] Indium sulfate significantly reduced the survival rate of V79 cells. After exposed to indium sulfate at various dosages for 12h, the mean intracellutar fluorescence intensity obviously increased from 21.60 to 31.10 while the Aψm significantly decreased from 0.191 3 to 0.1090. The statistical differences were significant between the control group and the exposure groups (P<0.05). [ Conclusion ] Indium sulfate can increase the release of intracellular ROS and reduce the A ψm significantly. The damage of indium sulfate to V79 cells might be associated with die change of normal cellular metabolism.关键词
硫酸铟/V79细胞/活性氧/线粒体膜电位Key words
Indium sulfate/ V79 cell/ reactive oxygen species/ mitochondrial membrane potential分类
医药卫生引用本文复制引用
赵静珺,肖卫..硫酸铟对V79细胞内活性氧和线粒体膜电位的影响[J].环境与职业医学,2011,28(11):684-686,3.
