安徽农业科学2011，Vol.39Issue(26)：15840-15842,3.
产脂肪酶真菌的筛选及产酶条件优化
Screening of Fungi Producing Lipase and Optimization of Its Lipase-producing Condition
摘要
Abstract
[Objective]The study aimed to seek for the fungi with high yield of lipase from the greasy soil, so as to provide the material for ex panding the fungi source of lipase. [ Method]22 strains of fungi producing lipase were isolated by enrichment culture and flat screening methods in soil samples collected from Shanghai City, Tangshan City of Hebei Province, Xinghua area of Jiangsu Province, Hefei area of Anhui Province. A stain with higher activity of producing lipase was isolated by re-screening methods. The strain was identified according to "The Common and Com mon-used Fungi" and the effects of different conditions on the strain producing lipase were studied. [Result] A stain with higher ability of produ cing lipase was isolated from the greasy soil. The strain was identified as Aspergillus niger according to groups characteristic and individuals forms of colonies and the relevant appraisal manual. The study on the conditions of producing enzyme for the strain showed that the optimal conditions of producing enzyme were as follows: the temperature for fermentation of 30 t, the initial pH of 7, the medium volume of 20% , and the optimum carbon and nitrogen sources were starches and peptones, resp. [Conclusion]The study laid a foundation for lipase producing bacteria and industri al production and application of lipase to some extent.关键词
脂肪酶/筛选/产酶条件Key words
Lipase /Screening/ Producing lipase condition分类
农业科技引用本文复制引用
李军红,姜绍通,童洋洋..产脂肪酶真菌的筛选及产酶条件优化[J].安徽农业科学,2011,39(26):15840-15842,3.基金项目
国家863项目(2010AA101503). （2010AA101503）
