摘要
Abstract
[Objective]To established a quantitative method of determination of protocatechuic acid in cortex acanthopanacis radicis. [Method] High proficiency liquid chromatography ( HPLC ) coupled with four-channel UV-visible detector was used, C,, chromatographic column, mobile phase was the buffer solution of methanol and sodium dihydrogen phosphate (0.1 mol/L) ,the volume ratio of methanol and sodium dihydrogen phosphate was 15=85 ,pH = 2.5. Qualitative evaluation was done by retention time and four-channel UV spectrum at the same time,and the detec tion wavelength were 245 nm,260 nm,275 nm,290 nm. Selected the best sensitive wave of 260 nm to make quantitative evaluation. Result:The linearity of protocatechuic acid was in the range of 0. 5 -6.0 ml/L (r =0.999 9) ,the average recovery was 99.87% ,the RSD was 2.11%. [Con clusion] The method is reliable,and can be used for quality control of cortex acanthopanacis radicis.关键词
高效液相色谱法/四通道紫外可见检测法/五加皮( CORTEX ACAN THOPANACIS)/原儿茶酸Key words
High proficiency liquid chromatography (HPLC)/Four-channel UV-visible detector/CORTEX ACANTHOPANACIS RADICIS/Protocatechuic acid .分类
农业科技
