厦门大学学报（自然科学版）2011，Vol.50Issue(5)：896-902,7.
重组大肠杆菌产β-1,3-1,4-葡聚糖酶的培养基优化
Medium Optimization for β-1,3-1,4-Glucanase Production by Recombinant Escherichia coli
摘要
Abstract
In order to improve the enzyme activity of Escherichia coli Rosetta (DE3)-pET-22-bgl-(kil-Km), the effects of carbon sources,nitrogen sources and C/N ratio on the production of β-1,3-l,4-glucanase H(A107-M) by E.coli Rosetta(DE3)-pET-22-bgl-(kil-Km) were investigated by one-factor-a-time experiment. Box-Behnken design and a response surface methodology (RSM) were further used to optimize the above critical factors for enzyme production. The optimized medium contained (g/L):glycerol 10. 01,casein peptone 12. 03,yeast 24,NaCl 10.(NH4)2SO4 4. 77,KH2PO4 2. 31,and K2HPO4 12. 54. When the recombinant E. Coli was cultivated in the optimized medium for 27 h,the enzyme activity of β-1,3-l,4-glucanase HCA107-M) reached 78. 32 U/mL (lichenan as the substrate), which was about 2. 84 times of which obtained in the initial medium.关键词
β-1,3-1,4-葡聚糖酶/重组大肠杆菌/培养基优化/响应面分析Key words
β-1,3-1,4-glucanasei/Escherichia coli/medium optimization/response surface methodology分类
生物科学引用本文复制引用
陈亚兰,黄伟强,周晓波,凌雪萍,卢英华..重组大肠杆菌产β-1,3-1,4-葡聚糖酶的培养基优化[J].厦门大学学报（自然科学版）,2011,50(5):896-902,7.基金项目
科技部科研院所技术开发研究专项资金(NCSTE-2007-JKZX-023) （NCSTE-2007-JKZX-023）
