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东方百合“索邦”离体培养技术探索

袁树先 陈海伟 刘珍梅

安徽农业科学2011,Vol.39Issue(26):15893-15895,3.
安徽农业科学2011,Vol.39Issue(26):15893-15895,3.

东方百合“索邦”离体培养技术探索

Study on in vitro Cultivation Technology of Lilium orential“Sorbonne”

袁树先 1陈海伟 1刘珍梅1

作者信息

  • 1. 赤峰学院生命科学系,内蒙古赤峰024000
  • 折叠

摘要

Abstract

[Objective] The research aimed to study in vitro cultivation technology of Lilium orential " Sorbonne". [ Method ] The lodicule of Lili um orential "Sorbonne" was as the explant.the influences of different hormone concentration ratios and squamous bulb parts on the adventitious bud induction were inspected. [Result] The bud induction effect of MS +2.0 mg/L of 6-BA +0.5 mg/L of NAA medium was the best,and the induction ratio was 60%. The bud induction capability of different squamous bulb part was middle > external > internal. Moreover,when the in duced adventitious bud was transferred into the rooting medium (1/2MS +0.1 mg/L of 6-BA +0.1 mg/L of NAA +0.2% of active carbon) ,the rooting rate could reach 100%. [Conclusion] The establishment of Lilium orential "Sorbonne" invitro regeneration system had the important sig nificance for reducing the dependence on the imported lily bulb and enlarging the lily production in China.

关键词

东方百合“索邦”/离体培养/鳞片

Key words

Lilium orential "Sorbonne"/ In vitro cultivation/ Lodicule

分类

农业科技

引用本文复制引用

袁树先,陈海伟,刘珍梅..东方百合“索邦”离体培养技术探索[J].安徽农业科学,2011,39(26):15893-15895,3.

基金项目

内蒙古教育厅高校科研项目(NJzy08151). (NJzy08151)

安徽农业科学

0517-6611

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