响应面法优化黑曲霉产木聚糖酶发酵条件的研究OA北大核心CSTPCD
Study on the Optimization of the Fermentation Conditions of Xylanase Prodution by Aspergillus niger Using Response Surface Methodology
从实验室保藏的10株黑曲霉菌株中经过筛选,得到一株产木聚糖酶活性较高的菌株黑曲霉F-3.利用响应面分析方法,对该菌株发酵产木聚糖酶的发酵温度、发酵时间和pH性值进行了优化研究,得到了产木聚糖酶的最优条件:发酵温度为30.8℃、发酵时间为98.7 h、起始pH为4.85,在该优化条件下,木聚糖酶的最高酶活可达到266.41 U/mL,较优化前提高了64.25％.
Aspergillus niger F - 3 with a higher activities of xylanase was screened from ten strains of Aspergil-lus sp. Preserved in the laboratory. Using response surface methodology, the fermentation conditions including fermentation temperature, fermentation time and initial pH were investigated, and the optimal conditions were obtained. The conditions are: fermentation temperature 30.8℃,fermentation time 98.7 h,starting pH 4.85. U/mL. Under these conditions, the …查看全部>>
马晓建;杨君芳;常春
郑州大学化工与能源学院,河南郑州450001郑州大学化工与能源学院,河南郑州450001郑州大学化工与能源学院,河南郑州450001
生物科学
黑曲霉木聚糖醇筛选优化响应面分析方法
Aspergillus nigerxylanasescreenoptimizationresponse surface methodology
《郑州大学学报（工学版）》 2011 (5)
30-33,4
国家科技支撑计划资助项目(2007BAD66B04)河南省教育厅自然科学研究计划项目(2008B480004)
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