华南农业大学学报2011，Vol.32Issue(4)：82-85,4.
mRNA差异显示技术研究中外猪种肉质性状差异表达基因
Study on the Different Expression of Meat Quality Candidate Genes Between the Local and Exotic Pig Breeds Using mRNA Differential Display Technology
摘要
Abstract
Comparative mRNA expression analysis of the ophthalmoplegia was made between Lantang ( one of the Chinese indigenous pig breeds) and Large White using mRNA differential display technology. Differential expression bands were recycled and re-amplified. Different expressions of five ESTss which were related to the meat quality traits were detected in the two pig breeds. ESTsp7 was expressed higher whereas ESTsp8, ESTsp9, ESTs10 and ESTsp11 were expressed lower in the Large White. Through the NC-BI, it was found that ESTsp7 and cytochrome oxidase subunit M gene in the range of 473 bp had 100% similarity. ESTsp9, ESTsp10, ESTsp11 and pig's SLN(Sarcolipin) gene had 94% -95% similarity. ESTsp8 was not matched with any other genes.关键词
猪/mRNA差异显示技术/肉质性状/ESTsKey words
pig/ mRNA differential display technology/ meat quality traits/ EST分类
农业科技引用本文复制引用
覃立立,童雄,徐建,张豪,李加琪,王翀..mRNA差异显示技术研究中外猪种肉质性状差异表达基因[J].华南农业大学学报,2011,32(4):82-85,4.基金项目
广东省自然科学基金重点项目(9251064201000005) （9251064201000005）
广东省科技计划项目(2008B020600009) （2008B020600009）
