热带作物学报2011，Vol.32Issue(7)：1201-1205,5.DOI:10.3969/j.issn.1000-2561.2011.07.003
华南5号和华南6号木薯体胚发生过程中酯酶和淀粉酶同工酶分析
Esterase and Amylase Isozymograms during Somatic Embryogenesis in Cassava (Manihot esculenta Crantz) SC5 and SC6
摘要
Abstract
The somatic embryos in duced from mature somatic embryo green cotyledon of two cassava cultivar SC5 and SC6. The slab polyacrylamide gel electrophoresis of esteraae (EST) and amylase isozyme were carried out with the explants cultured for 0, 5, 10, 20 and 30 d. The results showed that expression of esterase isozymes between two cassava cultivars (SC5 and SC6) were different. The mobility of electrophoretic band E2, E3 in SG5 was different to thai in SC6. These differences may be caused by variety Cassava Cultivars. In SC5, E3 was the strongest expression band during the development of somatic embryo, while in SC6, il was E'4 band. The differences of Amylase isozyme between SC5 and SC6 were that, three bands have been observed during the development of SC5 somatic embryos, while in SC6 there was only one band. When the explants were cultured for 5 d, the amylase got the strongest expression in SC5, while in SC6 it was got in the explants cultured for 0 and 20 d.关键词
木薯/体胚/酯酶/淀粉酶/同工酶Key words
Cassava/Somatic embryo/Esterase/Amylase/Isozyme分类
农业科技引用本文复制引用
赖杭桂,李瑞梅,王静,符少萍,胡新文,张鹏,郭建春..华南5号和华南6号木薯体胚发生过程中酯酶和淀粉酶同工酶分析[J].热带作物学报,2011,32(7):1201-1205,5.基金项目
国家973重大基础性研究项目(No.2010CB126600):国家自然科学基金No.30860151 （No.2010CB126600）
中央级公益性科研院所基本科研项目NoITBBZD0721 （）
国家863计划(No.2009AA10Z102) （No.2009AA10Z102）
海南省普通高等学校研究生创新科研课题(Hxwby008-08). （Hxwby008-08）
