广东海洋大学学报2011，Vol.31Issue(4)：62-68,7.
水产饲料中三聚氰胺检测方法的优化
Optimization of Testing Methods of Melamine Residues in Aquatic Feed
摘要
Abstract
Using aquatic feed as subjects, the best testing method of melamine residues were set up according to the optimal chromatographic and pre-treatment conditions by HPLC. The results showed that the optimum detecting condition was that the mobile phase was composed of 92% sodium heptanesulfonate and 8% acetonitrile, temperature was 40°C, flow rate 1.2mL/min and wavelength 236nm. The optimum extraction conditions was that extract solution included 18mL 50% acetonitrile, lmL zinc acetate and lmL Imol/L HC1, leacheate was 3mL water and 3mL methyl alcohol, elutionvolume 6mL. The coefficient of recovery of the method reached 85% ~ 100%, RSD was less than 5%, standard working line linear ranged from 0.2 mg/L to 5.0 mg/L, correlation coefficient was 0.9998, the detection limit of melamine 0.2 mg/kg.关键词
水产饲料/三聚氰胺/高效液相色谱法Key words
aquatic feed/ melamine/ High performance liquid chromatographic分类
农业科技引用本文复制引用
黄和,黄春丽,陈荷英,高平,罗林,陈宏..水产饲料中三聚氰胺检测方法的优化[J].广东海洋大学学报,2011,31(4):62-68,7.基金项目
广东省科技计划项目(2009B020316002) （2009B020316002）
