广东海洋大学学报2011，Vol.31Issue(4)：6-12,7.
荷那龙罗非鱼两种GHSR基因的克隆与序列分析
Molecular Clone and Sequence Analysis of GHSR Gene of Zanzibar Tilapia (Oreochromis hornorum)
摘要
Abstract
Two cDNA transcripts, namely GHSR-la and GHSR-lb, for growth hormone secretagogue receptor (GHSR) were identified from the Zanzibar tilapia (Oreochromis hornorum) pituitary by RT-PCR and RACE method. The GHSR-la, 1 627 bp in length, consists of a 225-bp 5'-untranslated region, a 266-bp 3'-untranslated region and a 1 155-bp open reading frame (ORF) encoding 384 amino acids and including seven transmembrane domains (TM). The GHSR-lb, 1 877 bp in length, consists of a 225-bp 5'-untranslated region, a 755-bp 3'-untranslated region and a 897-bp ORF encoding 298 amino acids and including five TM. Comparison between cDNA and gene sequences of GHSR showed that the two transcripts were two alternative splicing forms of a single GHSR gene.关键词
荷那龙罗非鱼/生长激素促分泌素受体( GHSR)/基因克隆/基因序列Key words
Oreochromis hornorum/ growth hormone secretagogue receptor (GHSR)/ gene cloning/ gene sequences分类
生物科学引用本文复制引用
高风英,王欢,叶星,卢迈新,黄樟翰,朱华平..荷那龙罗非鱼两种GHSR基因的克隆与序列分析[J].广东海洋大学学报,2011,31(4):6-12,7.基金项目
现代农业产业技术体系建设专项资金(CARS-49),广东省农业重点项目(2009B020201003),广东省海洋渔业科技推广专项(A200901C01) （CARS-49）
