安徽农业科学2011，Vol.39Issue(26)：16061-16062,16103,3.
嗜热厌氧杆菌X514在不同氮源培养基中的生长及代谢研究
Study of Growth and Metabolism of Thermoanaerobacter sp.X514 under Different Nitrogen Sources
摘要
Abstract
[Objective] The aim was to find out the appropriate nitrogen medium supplement for Thermoanaerobacter sp. X514. [ Method ] After comparing the growth and mechanism of X514 in media added with different nitrogen medium supplements (including the organic medium supple ments : trptone and yeast extract and inorganic medium supplement NH4Cl),a suitable medium supplement for X514 was found. [Result] The grwoth results in various media added with 5 g/L glucose showed that trptone and yeast extract could promote the growth of X514 compared to NH4C1; the maximum growth rate was 0.15 h- (the basal medium added with 5 g/L yeast extract); and the growth of X514 in the medium with high concentration of trytone(5 g/L) was delayed for4 hours compared to it in the medium with lower concentration of tryptone(0.5 g/L). Final ly , after analyzing the product distribution in those media, yeast extract was more effective in promting the ethanol production and the ethanol con versation rates(mol ethanol produced/ mol glucose consumed) in media added with yeast extract was 30% more than those in media added with equal amount of tryptone on average. [ Conclusion]Through those experiments,it was found that compared with the equal amount of tryptone,yeast extract was more effective in promoting the growth and ethanol production of X514.关键词
嗜热厌氧杆菌/蛋白胨/酵母提取物Key words
Thermoanaerobacter/ Tryptone/Yeast extract分类
资源环境引用本文复制引用
谢天..嗜热厌氧杆菌X514在不同氮源培养基中的生长及代谢研究[J].安徽农业科学,2011,39(26):16061-16062,16103,3.基金项目
国家自然科学基金项目(30800202). （30800202）
