热带作物学报2011，Vol.32Issue(7)：1304-1308,5.DOI:10.3969/j.issn.1000-2561.2011.07.023
巴西橡胶树胚胎晚期丰富蛋白基因的克隆及序列分析
Cloning and Sequence Analysis of the Late Embryogenesis Abundant Related Protein Gene from Hevea brasiliensis
摘要
Abstract
Using the primers designed from the known EST from a suppression subtractive hybridization-cDNA library, the full-length cDNA sequence of HbLEA 14 was cloned from Hevea brasiliensls latex by 3'-RACE technique. The opening reading frame (ORF) of HbLEA 14 was 456 bp in length, encoding a 151-amino-acid polypeptide with a predicted molecular weight of 16.58 ku and isoelectric point of 5.10. Cluster analysis revealed that HbLEA14 was an atypical LEA2, and showed 88% identity with Ricinus communis RcLEA14, 80% to Euphorbia esula EeLEA14, 75% to Gossypium hirsutum GhLEA14, 73% to Glycine max GmLEA14, 65% to Arabidopsis thaliana AtLEA14 and 61% to Solanum lycopersicum S1ER5, respectively. In addition, HbLEA 14 exhibited higher transcriptional expression level in the latex of tapping panel dryness trees than in healthy trees. This paper will benefit to further analyze the functions of HbLEA 14.关键词
巴西橡胶树/胚胎晚期丰富蛋白/基因克隆/序列分析/RT-PCRKey words
Hevea brasiliensis/LEA protein/Gene cloning/Sequence analysis/RT-PCR分类
农业科技引用本文复制引用
喻修道,王真辉,袁坤,杨礼富..巴西橡胶树胚胎晚期丰富蛋白基因的克隆及序列分析[J].热带作物学报,2011,32(7):1304-1308,5.基金项目
中国热带农业科学院橡胶研究所基本科研业务费专项资金资助项目(No.YWFZX2010-9,No.1630022011014). （No.YWFZX2010-9,No.1630022011014）
