畜牧与兽医2011，Vol.43Issue(10)：5-8,4.
牛皮蝇蛆病间接ELISA诊断方法的建立
Establishment of an indirect ELISA for the detection of hypodermiosis in cattle
摘要
Abstract
An indirect ELISA for hypodermiosis was established with antigens prepared from the first instar larvae of Hypoderma lineatum. The ideal serum concentration of 80 dilutions and the antigen coating concentration of 26. 64 μg/mL were obtained by square matrix experiment , and then the specificity, sensitivity and repeatability of the diagnostic method were tested. 233 bovine sera from the Inner Mongolia region were tested by the indirect ELISA. The results demonstrated that the established indirect ELISA for hypodermiosis with higher specificity, sensitivity and repeatability, could be used for the serological investigation of hypodermiosis.关键词
牛皮蝇蛆病/虫体抗原/间接ELISAKey words
hypodermiosis/ larvae antigen/ indirect ELISA分类
农业科技引用本文复制引用
王文龙,阮风雷,刘春霞,初一鑫,呼和巴特尔..牛皮蝇蛆病间接ELISA诊断方法的建立[J].畜牧与兽医,2011,43(10):5-8,4.基金项目
教育部“春晖计划”合作项目(Z2007-1-01018) （Z2007-1-01018）
国家自然科学基金(30360082). （30360082）
