畜牧与兽医2011，Vol.43Issue(10)：9-12,4.
抗猪(嗜血)支原体MSG1蛋白单克隆抗体的制备及鉴定
Preparation and identification of monoclonal antibodies against MSG1 protein of Mycoplasma suis
摘要
Abstract
The monoclonal antibodies (Mabs) were prepared by fusing mouse myloma cells (SP2/0) with spleen cells from BALB/c mice immunized with purified recombinant MSG1 protein of Mycoplasma suis. Two hybridoma cell lines secreting Mabs against MSG1 protein were screened by indirect enzyme-linked immunosorbent assay (ELISA) and named as 1A7 and 3G6, respectively. Western-blot results showed that the two Mabs reacted specifically with recombinant MSG1 protein. Their ELISA titers in supernatant and asicite were 1 : 4096, 1 : 1024 and 1 : 1638400, 1 : 51200, respectively. The isotypes of 1A7 and 3G6 both belong to IgGl/n and the additive ELISA showed that the two Mabs reacted with the same antigen determinant. So, we concluded that the specific anti-MSGl protein Mabs were developed and these Mabs may be useful in the development of detection methods and provide a basis for investigating the pathogenicity of M. Suis.关键词
猪嗜血支原体/MSG1蛋白/单克隆抗体Key words
Mycoplasma suis/ MSG1 protein/ monoclonal antibodies分类
农业科技引用本文复制引用
张长莹,李玉峰,曹彦琼,宋艳华,陈闻,姜平..抗猪(嗜血)支原体MSG1蛋白单克隆抗体的制备及鉴定[J].畜牧与兽医,2011,43(10):9-12,4.基金项目
公益性行业(农业)科研专项项目(200903036-10). （农业）
