畜牧与兽医2011，Vol.43Issue(10)：25-30,6.
藏系绵羊VEGF基因的克隆、同源性分析及其表达水平的实时荧光定量PCR检测
Cloning, homology analysis and expression level of the VEGF gene by a real-time fluorescent quantitative PCR in Tibetan sheep
摘要
Abstract
The VEGF gene was amplified from the skin of Tibetan sheep by PCR, 573 bp in length and coded 190 araino acids. Our results indicated that the homology of VEGF gene between Tibetan sheep and some other species was from 100% to 74. 5% , in which the similarity of the gene sequence of Tibetan sheep and ordinary sheep was 100% , 99. 1% and 96. 2% similar to cattle and pigs, but compared with chicken, the homology was 74.5%. The expression level of VEGF gene from Tibet sheep skin cells were analyzed by using fluorescence quantitative PCR SYBR Green I Fluorescent dyes technology. The results indicated that the expression level of VEGF gene on lamb was 11.5 and 0. 85 times, respectively in the Tibetan pregnancy female sheep groups fed with Nutrition Brick and Granule Feed compared with that of control group. To improve the expression level of VEGF gene in the lamb, it is better to feed Nutrition Brick to Tibetan pregnancy female sheep than Granule Feed.关键词
藏系绵羊/VEGF基因/克隆/同源性分析/实时荧光定量PCRKey words
Tibetan sheep/ VEGF gene/ clone/ homology analysis/ real time fluorescent quantitative PCR分类
农业科技引用本文复制引用
窦全林,杨发龙,白文林,马志杰,尹荣焕,陈刚..藏系绵羊VEGF基因的克隆、同源性分析及其表达水平的实时荧光定量PCR检测[J].畜牧与兽医,2011,43(10):25-30,6.基金项目
青海省科技厅资助项目(2005-N-159). （2005-N-159）
