浙江农林大学学报2011，Vol.28Issue(5)：815-822,8.
濒危树种金钱松RAPD体系的建立和遗传多样性分析
RAPD analysis of genetic diversity for Pseudolarix amabilis:a critically endangered plant
摘要
Abstract
In order to protect germplasm resources of endangered plant: Pseudolarix amabilis, genetic diversity of Pseudolarix amabilis from different sources in Zhejiang Province were analyzed using an optimal protocol of random amplified polymorphism DNA (RAPD) followed by a cluster analysis in this study. Results showed that for 62 individuals with 18 random primers, 172 repetitive loci were detected, among which 170 were polymorphic for a species level polymorphic loci rate of 99.2%. The cluster analysis showed that materials from the same sources were classified together. Thus, RAPD polymorphism classification for natural populations of P. Amabilis was mostly related to geographic distribution, genetic diversity was high among the P. Amabilis population, and both in situ preservation and ex-situ conservation were possible.[Ch, 2 fig.3 tab.31 ref.]关键词
林木育种学/金钱松/遗传多样性/随机扩增多态性DNA (RAPD)/迁地保护Key words
forest tree breeding/Pseudolarix amabilis/genetic diversity/random amplified polymorphism DNA/ex-situ conservation分类
农业科技引用本文复制引用
高燕会,樊民亮,骆文坚,黄华宏,童再康..濒危树种金钱松RAPD体系的建立和遗传多样性分析[J].浙江农林大学学报,2011,28(5):815-822,8.基金项目
浙江省科技计划项目(2006C12059-4 （）
2006C22064) （）
