安徽农业科学2011，Vol.39Issue(26)：16139-16140,16163,3.
饲料中沙门氏菌的分离鉴定与Dot-ELISA检测
Detection Salmonella in Forage by Dot-ELISA
韩志辉 1张红见1
作者信息
- 1. 青海大学农牧学院动物医学系,青海西宁810016
- 折叠
摘要
Abstract
[Objective] To develop a Dot-ELISA ( Dot-enzyme linked immunosorbent assay) method for detection of Salmonella in forage. [ Method ] Forage samples including 100 finished feed samples, 85 fishmeal samples and 50 protamine samples were randomly collected from a feed factory in Xining City, and Salmonella in these samples were detected by Dot-ELISA. The Salmonella isolates were further identified using biochemical tests and serological tests. [Result] As detected by Dot-ELISA, the positive rates of Salmonella in the finished feed, fishmeal and protamine were 38.00% , 62.35% and 60.00% , respectively. The coincidence rate between the Dot-ELISA and routine identification method was 82.23%. [Conclusion] The Dot-ELISA has good specificity and sensitivity and is highly coincidental with the routine identification method. Therefore, Dot-ELISA can be used for detection of Salmonella in forage.关键词
Dot-ELISA/沙门氏菌/检测/饲料Key words
Dot-enzyme linked immunosorbent assay/ Salmonella/ Detection/ Forage分类
农业科技引用本文复制引用
韩志辉,张红见..饲料中沙门氏菌的分离鉴定与Dot-ELISA检测[J].安徽农业科学,2011,39(26):16139-16140,16163,3.
