农业工程学报2011，Vol.27Issue(10)：370-374,5.DOI:10.3969/j.issn.1002-6819.2011.11.065
研磨珠辅助提取茶树菇水溶性核苷酸工艺优化
Process optimization of assisted extraction of water-soluble nucleotides from Agrocybe cylindracea by grind bead
程翠林 1王振宇 1赵海田 2崔茉莉 1姚磊1
作者信息
- 1. 哈尔滨工业大学食品科学与工程学院,哈尔滨150090
- 2. 东北林业大学林学院,哈尔滨150040
- 折叠
摘要
Abstract
The optimum conditions for extraction and separation of water-soluble nucleotide from fruit body of an edible fungus-Agrocybe cylindracea was studied. The crude nucleotides were assisted extraction with the grind bead . Three factors (grind time, ratio of liquid to solid, and loading capacity of grind beads) were investigated in the single-factor experiments. The process parameters were optimized by response surface analysis method. A multiple regression equation which was identified significant by regression analysis and the optimum extraction conditions were also determined (grind time: 12min, ratio of liquid to solid: 51:1 Ml/g, loading capacity of grind beads: 12.5%). The theoretical yield for nucleotide was 7.02%. Furthermore, the actual yield was 6.94%, which was in accordance with theoretical result and thereby illustrated the fitted multiple regression equation was credible. Compared with traditional methods, grind bead assisted extraction not only produced high yield of nucleotides, but also greatly shortened processing time, which provided an industrial reference for developing new processes of nucleotides production from edible fungi.关键词
优化/提取/核苷酸/研磨珠/茶树菇/响应面分析Key words
optimization/ extraction/ nucleotide/ grind bead/ Agrocybe cylindracea/ response surface analysis分类
轻工纺织引用本文复制引用
程翠林,王振宇,赵海田,崔茉莉,姚磊..研磨珠辅助提取茶树菇水溶性核苷酸工艺优化[J].农业工程学报,2011,27(10):370-374,5.
