中国组织工程研究与临床康复2011，Vol.15Issue(44)：8175-8178,4.DOI:10.3969/j.issn.1673-8225.2011.44.002
肝移植后缺血再灌注损伤大鼠诱导细胞凋亡中的一氧化氮
Effects of nitric oxide on cell apoptosis induced by ischemia/reperfusion injury after liver transplantation in rats
摘要
Abstract
BACKGROUND: Nitric oxide secreted by mducibte nitric oxide synthass is one of important factors in the pathophysiologicalchanges of the liver graft caused by Ischemia/reperfusion injury.OBJECTIVE: To investigate the effects of nitric oxide on the cell apoptosis Induced by ischemia/reperfusion injury after livertransplantation In rate and the gene expression of caspase-3.METHODS: Seventy-two recipient rats were randomly divided into transplantation, arginine and L-NAME groups, and they weredeveloped into rat models of orthotopic liver transplantation. At 5 minutes before surgery, arginine, which can increase serumlevel of nitric oxide, and L-NAME, an inhibitor of nitric oxides were injected into the arginine and L-NAME groups, respectively.After model establishment, the remaining 24 rats were included into sham-surgery group.RESULTS AND CONCLUSION: The activity of serum transaminase in the transplantation group was significantly higher than inthe arginine group, but it was significantly lower than in the L-NAME group (P< 0.01). Serum level of nitric oxide in thetransplantation group was higher than in the L-NAME group, but it was lower than in the arginine group. Earty cell apoptosispeaked at 3 hours after reperfusion. The percentage of living hepatocytes and the expression of caspase-3 In the transplantationgroup were higher than in the arginine group, but it was lower than in the L-NAME group. These findings suggest that nitric oxideexhibits protective effects on earry apoptosds of rtepatocytes-induceo by ischemisireperfusion after orthotopic river transplantation,which may be mediated by downregulating caspase-3 gene expression.关键词
一氧化氮/肝移植/缺血再灌注损伤/凋亡/基因表达分类
医药卫生引用本文复制引用
刘其雨,李立,李晓延,陈刚,赵英鹏,白建华,朱新锋..肝移植后缺血再灌注损伤大鼠诱导细胞凋亡中的一氧化氮[J].中国组织工程研究与临床康复,2011,15(44):8175-8178,4.基金项目
云南省科技计划项目(2008CD195),课题名称:CYP3A5基因型在肝移植供受体匹配及术后FK506用药中的作用研究.昆明市科技计划项目(08S100304-2),课题名称:昆明器官移植研究中心建设. （2008CD195）
