中国组织工程研究与临床康复2011，Vol.15Issue(44)：8325-8328,4.DOI:10.3969/j.issn.1673-8225.2011.44.039
辐照灭菌冷冻干燥羊膜修复碱烧伤
Transplantation of freeze-dried amnion after irradiation sterilization for corneal alkali burn
滕丹 1栾新尧 1许妮娜 1于丽萍 1李文姬 1刘艳红 1何伟2
作者信息
- 1. 沈阳绿谷生物技术产业有限公司,辽宁省沈阳市,110163
- 2. 沈阳何氏眼科医院,辽宁省沈阳市110034
- 折叠
摘要
Abstract
BACKGROUND: The freeze dried amnion after radiation sterilization is easy to keep and use. However, whether the amnion'sactivity will be reduced and clinical effect will be affected after these treatments have been rarely reported.OBJECTIVE: To evaluate the repair function of freeze-dried amnion after radiation sterilization on rabbit cornea.METHODS: Nine healthy adult rabbits were taken and their corneas were burned with 1 mold NaOH. The left corneas served astest group and the right corneas as control group. The freeze-dried amnion was planted into the test group but not planted into thecontrol group. Within 30 days after surgery, cornea! Clearness, neovasculanzation and recovery of upper cornea were dailyobserved through the use of slit lamp.RESULTS AND CONCLUSION: At 30 days after surgery, comeal clearness, neovasculanzation and recovery of upper corneawere significantly better in the test group than in the control group (P< 0.05). Compared wpth the control group, upper cornea wasbetter recovered, matrix fibers were more likely in order, infiltration of inflammatory cells was slighter, and there were fewer newlyformed vessels in the test group. These findings suggest that freeze-dried, irradiated amnion can alleviate inflammatory response,promote recovery of upper cornea and reduce neo-vascularization after transplantation into alkali burned rabbit cornea.关键词
冷冻干燥羊膜/碱烧伤/辐照灭菌/角膜/炎性反应/组织构建分类
医药卫生引用本文复制引用
滕丹,栾新尧,许妮娜,于丽萍,李文姬,刘艳红,何伟..辐照灭菌冷冻干燥羊膜修复碱烧伤[J].中国组织工程研究与临床康复,2011,15(44):8325-8328,4.
