中国组织工程研究与临床康复2011，Vol.15Issue(44)：8272-8276,5.DOI:10.3969/j.issn.1673-8225.2011.44.026
维持性血液透析患者外周血单个核细胞核因子kB活性及临床意义
Nuclear factor-kappa B activity in peripheral blood mononuclear cells from patients undergoing maintenance hemodialysis and its clinical significance
摘要
Abstract
BACKGROUND: The nuclear factor-kB (NF-kB) activity in peripheral blood mononudear cells (PBMCs] from patients undergoing maintenance hemodialysis (MHD) before renal transplantation has been found to be increased abnormally. OBJECTIVE: To investigate NF-kB activity in PBMCs from patients underlying MHD before renal transplantation and its correlation with high sensitive C-reactive protein (hs-CRP) levels and malnutrition.METHODS: The nutritional status, anthropometry indicators, blood biochemistry, the NF-kB p65 activity in PBMCs and senjm hs-CRP level in 71 patients undergoing MHD before renal transplantation and healthy controls were detected. MHD patients were divided into non-inflammatory group (< 3 mg/L) and slight inflammation group (a 3 mg/L) according to me hs-CRP levels. RESULTS AND CONCLUSION: NF-kB activity in PBMCs and serum hs-CRP levels were significantly higher in MHD patients than in healthy controls (P < 0.001}. The number of malnutrition patients, the score of modified quantitative subjective global assessment of nutrition (MQSGA), and the NF-kB activity in PBMCs as well as serum hs-CRP level were significantly higher in the slightly inflammatory group than in the non-inflammatory group (P≤0.001 or P < 0.01 or P < 0.05). The hemoglobin, serum albumin, serum prealbumin, mid-arm muscle circumference, mid-arm circumference and grasp force were all significantly lower in the non-inflammatory group than In the non-inflammatory group (P≤0.001) and the NF-kB activity in PBMCs were sign ificantJy higher in the non-inflammatory group than in the healthy control group (P≤ 0.001). There was no significant difference in serum hs-CRP levels between non-inflammatory group and healthy control group {P > 0.05). Results showed that NF-kB was abnormally activated in PBMCs in patients underlying MHD before renal transplantation; with worsened inflammatory reaction, serum hs-CRP levels and the abnormal activation of NF-kB would be increased and the degree of malnutrition would be more serious. These finding demonstrates that the slight inflammatory status could be detected and malnutrition could be predicted earlier according to NF-kB activity in PBMCs than serum hs-CRP levels.关键词
血液透析/核因子kB/超敏C-反应蛋白/营养不良/单个核细胞分类
医药卫生引用本文复制引用
王景福,张昆,胡桂才,徐素欣,郭风玲,赵亚娟,段书众,朱菊平,邢恩鸿..维持性血液透析患者外周血单个核细胞核因子kB活性及临床意义[J].中国组织工程研究与临床康复,2011,15(44):8272-8276,5.基金项目
承德市科学技术研究与发展计划(自筹经费)项目(201021051). （自筹经费）
