重庆医学2012，Vol.41Issue(1)：4-7,封2,5.DOI:10.3969/j.issn.1671-8348.2012.01.002
口服MHC-CTB交联物对大鼠移植肾存活的影响
The effect of MHC-CTB cross-linking agent administrated orally on kidney allograft survival in rats
摘要
Abstract
Objective To observe the effect of MHC-CTB cross-linking agent administrated orally on kidney allograft survival in rats. Methods Synthesizing MHC peotides by using solid phase method, its molecular weight and purity were dectected. Oral toler ance was induced by intragastric administration (gavage) at a dose of 5 mg/kg twice weekly until day 60, while rats in control group feed with 1 ml. Physiological saline as conrol. BN(RTln) and Lewis (RT11) were chosed as donors and receipents, the allogenic kidney transplantation was performed,then the assays of CD4 CD25 T cells in peripheral blood;the survival time and the patho logical of kidney allograft was observed. The function of MHC inhibition to the proliferation of T cells was further observed by mix lymphocyte culture. Results The molecular weight and purity of MHC peptide was 1 389. 81 and 98. 1% respectively. The assays of CD4 + CD25 + T cells in peripheral blood were increased in experiment group at day 1 post-transplantation(P<0. 01) ;the survival time of kidney allografts in experiment group and control group were (21.4 + 1.45)d and (8. 2 + 2. 15)d respectively(P<0. 01). And transplantation kindey HK showed acute rejection happened earlier in the control. In vitro,the proliferation of T cells were in hibited and the effect of inhibition was highly increased by adding MHC peptides(P<0. 01). Conclusion Oral adminsitration of MHC peptides can induce immune tolerance? Inhibit the rejeciton of allograft kindey and prolong the survival time of kidney al lografts.关键词
移植/器官排斥/交联/口服耐受Key words
transplantation/allograft rejection/cross-linking/oral tolerance引用本文复制引用
余雪松,黄赤兵,范明齐,王平贤,冯嘉瑜,肖亚..口服MHC-CTB交联物对大鼠移植肾存活的影响[J].重庆医学,2012,41(1):4-7,封2,5.基金项目
国家自然科学基金资助项目(30872580). （30872580）
