重庆医学2012，Vol.41Issue(1)：11-13,封2,4.DOI:10.3969/j.issn.1671-8348.2012.01.004
激光捕获显微切割技术研究胃癌hMSH2基因突变与蛋白表达的关系
The study of expression and mutation of gene hMSH2 and correlation of them in gastric carcinoma
摘要
Abstract
Objective To study the expression and mutation of mismatch repair gene hMSH2 and correlation of them in gastric carcinoma,to evaluate their clinicapathological characteristics and the possible mechanism of carcinogenesis caused by hMSH2. Methods (1) 45 cases of gastric carcinoma were examed. The expression of hiVISriZ protein was examed by immunohistochemis try. (2)The cells of gastric carcinoma were got through laser capture microdissection. (3)45 cases of gastric carcinomas were stud ied respectively with five exons of hMSH2 gene emutation by using polymerase chain reaction(PCR) ,single strand conformation polymorphism(SSCP) and silver staining. Results (1) In 45 cases of gastric carcinoma, the negative expression rate of hMSH2 protein was 20. 00% while the positive expression rate was 80. 00%. No significant association was observed between hMSH2 pro tein expression and patient's age,gender,part,lymph node metastases,histological type,differentiation and stage of pathology(P> 0. 05). (2) In 45 cases of gastric carcinoma,five exons mutation rate of hMSH2 gene was 8. 89% and not significantly associated with gender,age,part,lymph node metastases and histologic type,differentiated grade and stage of clinical pathology(P>-0. 05). (3)The hMSH2 gene mutation rates of the gastric carcinoma with the hMSH2 protein negative expression and with the hMSH2 protein positive expression were 44. 44% and 0. 00% respectively. There were significant difference between them(P-<0. 01). Con clusion hMSH2 gene mutation may be involved into the pathogenesis of gastric carcinoma and cause depressed expression of hM SH2 protein.关键词
胃肿瘤激光捕获显微切割技术/hMSH2基因/蛋白表达/基因突变/免疫组织化学Key words
stomach neoplasms/ laser capture microdissection/ hiVISri2 gene/ mutation/ immunohistochemistry引用本文复制引用
刘连,王丽珍,李春鸣..激光捕获显微切割技术研究胃癌hMSH2基因突变与蛋白表达的关系[J].重庆医学,2012,41(1):11-13,封2,4.基金项目
贵州省优秀青年科技人才培养计划基金资助项目[黔科合人字(2005)0512号]. （2005）
