老年医学与保健2011，Vol.17Issue(6)：336-339,343,5.DOI:10.3969/j.issn.1008-8296.2011-06-06
细胞衰老影响MTT法药效评价及其改进方法
Impact of cellular senescence on drug efficacy evaluation by MTT assay and its improved methods
摘要
Abstract
Objective MTT assay is a conventional method to detect the growth-inhibitory effect. But sometimes there are some false-positive results. This study was to explore the causes of false-positive results from the cellular senescence. Methods HeLa and MCF-7 were detected by MTT, SRB, trypan Blue-stained counting and colony formation assay after exposure to bleomycin and boningmycin which induced cellular senescence in human tumor cells. Cellular senescence was detected with senescence-associated beta-galactosidase staining. Results The IC60 value measured by MTT assay was the largest and the IC50 by clone formation assay was the most sensitive, reaching nanomolar level. Conclusions Cellular sen-escence affects the accuracy of the results of MTT assay. Colony formation assay is the most reliable method to overcome the effects of cellular senescence.关键词
细胞衰老/药效评价/MTT法/博来霉素类/抗肿瘤药物Key words
Cellular senescence/ Drug efficacy evaluation/ MTT method/ Bleomycin family/ Antitumor agents分类
医药卫生引用本文复制引用
陈建国,张秀敏,陈汝贤,何琪杨..细胞衰老影响MTT法药效评价及其改进方法[J].老年医学与保健,2011,17(6):336-339,343,5.基金项目
国家"重大新药创制"科技重大专项大平台项目(2012ZX09301002-001)和微生物药物技术创新与新药创制产学研联盟项目(2010ZX09401-403) （2012ZX09301002-001）
