重庆医学2011，Vol.40Issue(36)：3705-3707,封3,4.DOI:10.3969/j.issn.1671-8348.2011.36.029
犬心房肌细胞分离及其L型钙通道电流检测的研究
A study on separation of canine atrial myocytes and detection of their L-type calcium channel current
邹帅 1龙毅 1范晋奇 1周亚南 1高大中 1殷跃辉1
作者信息
- 1. 重庆医科大学附属第二医院心血管内科,400010
- 折叠
摘要
Abstract
Objective To explore a stable and reliable method of canine atrial myocytes separation for improving the success rate of patch-clamp techniques and detecting the L-type calcium channel current. Methods Modified Langderoff perfusion system was adopted to perform retrograde aortic perfusion and selective coronary artery catheterization was used to obtain single atrial myot-cytes. Microscope was employed to observe the cellular morphology and the L-type calcium channel current was measured by whole-cell patch-clamp recording. Results The survival rate of separated cells was 70% to 80% ,and it was 30% to 40% after recalcifica-tion. A stable L-type calcium channel current could be recorded in calcium-tolerant cells which was rod-shaped with good refraction and clear horizontal stripe. Conclusion Modified Langderoff technique contribute to stable separation of canine atrial myocytes with high survival rate and normal electrophysiological properties,and can be used in the research of the iron channel of atrial myocytes.关键词
肌细胞,心脏/细胞分离/膜片钳术/钙通道,L型/犬Key words
myocytes,cardiac/cell separation/patch-clamp techniques/calcium channels, L-type/canine引用本文复制引用
邹帅,龙毅,范晋奇,周亚南,高大中,殷跃辉..犬心房肌细胞分离及其L型钙通道电流检测的研究[J].重庆医学,2011,40(36):3705-3707,封3,4.
