中国药理学通报2012，Vol.28Issue(1)：132-135,4.DOI:10.3969/j.issn.1001-1978.2012.031.
依达拉奉对LPC所致兔胸主动脉内皮功能的影响及机制
Effect of edaravone on LPC-induced vascular endothelial function in rabbit thoracic aorta and its mechanism
摘要
Abstract
Aim To investigate the effect of edaravone ( Eda ) on damage of vascular endothelium induced by lysophosphatidylcholine ( LPC ) in rabbit aorta and its mechanism. Methods The endothelium-dependent relaxation response to acetylcholine and endothelium-in-dependent relaxation response to sodium nitroprusside of aortic rings were measured by recording isometric tension after the rings were exposed to LPC( 5 mg ? L-1 )in the absence or presence of Eda( 25 ~ 100 μmol ? L -1 )to estimate injury effect of LPC and the protective effect of Eda on aortic endothelium, respectively. Furthermore, the content of nitric oxide( NO )and mal-onaldehyde( MDA ), and the activity of superoxide dis-mutase( SOD )in the thoracic aorta rings were measured after tension test. Results Exposure of aortic rings to LPC ( 5 mg ? L-1 ) for 30 min induced a significant inhibition of endothelium-dependent relaxation to acetyl-choline, but did not affect endothelium-independent relaxation to sodium nitroprusside. Pre-incubation of aortic rings with Eda for 15 min and then co-incubation of the rings with LPC ( 5 mg ? L ~ * ) for another 30 min significantly attenuated the inhibition of endothelium-dependent relaxation induced by LPC. LPC markedly decreased the activity of SOD and the level NO but significantly increased the content of MDA in vascular tissues. However, Eda significantly increased the level of NO and the activity of SOD but significantly decreased the content of MDA in vascular tissues. Conclusion Eda may protect endothelium from being damaged by LPC. The mechanism of Eda may be related to its an-tioxidant defenses.关键词
依达拉奉/溶血磷脂酰胆碱/内皮依赖性舒张/丙二醛/一氧化氮/超氧化物歧化酶/兔Key words
edaravone/ lysophosphatidylcholine/ endothelium-dependent relaxation/ malondialdehyde/ nitric oxide/ superoxide dismutase/rabbit分类
医药卫生引用本文复制引用
邓华菲,何汉江..依达拉奉对LPC所致兔胸主动脉内皮功能的影响及机制[J].中国药理学通报,2012,28(1):132-135,4.基金项目
湘南学院重点学科基金项目资助(No 201130) （No 201130）
