中国循证儿科杂志2012，Vol.7Issue(1)：31-36,6.DOI:10.3969/j.issn.1673-5501.2012.01.006
2009至2010年南京儿童医院5岁以下儿童病毒性腹泻分子流行病学特点
Molecular epidemiological study on viral diarrhea among pediatric patients under five years old in Nanjing, 2009-2010
摘要
Abstract
Objective A molecular epidemiological study on rotavirus( HRV), human calicivirus( HuCV), astrovirus ( AstV ) and adenovims( AdV ) was conducted in stool specimen from patients with diarrhea under 5 years of age in Nanjing Children Hospital of Nanjing Medical University from July 2009 to June 2010, to provide the basic data and theory evidence for prevention and treatment of viral diarrhea. Methods In Nanjing Children's Hospital of Nanjing Medical University from July 2009 to June 2010, 300 stool specimens were collected from outpatients under 5 years of age diagnosed as diarrhea. HRV was detected by ELISA, strain characterization was carried out by nested PCR. HuCV ( including norovirus and sapovirus ), AstV and AdV were detected by RT-multiplex PCR or PCR. Results In the 300 children whose samples were collected, 188 patients were boys, 112 were girls. The age of children ranged from 1 to 59 months ( mean ±SD age: 10. 5 ±9. 2 months ). At least one of the four viral agents was found in 60. 0% ( 180/300 ) of the specimens. HRV, HuCV, AstV and AdV were identified in 37. 7% ( 113/300 ), 23.7% (71/300) , 4. 0%( 12/300), and 2.7%( 8/300 ) in 300 specimens respectively. ①HRV G3 (38.9% ) was the most prevailing serotype, followed by G2 ( 8. 9% ). Based on P typing, P[ 8 ] ( 33. 6% ) was the most common genotype. The most common G-P combination was G3P[ 8 ] ( 15. 9% ). The incidence of HRV infections peaked from October 2009 to January 2010, HRV was mostly found in children aged from 7 to 12 months. ②Of 71 HuCV positive samples, norovirus was detected in 67 cases and sapovims was detected in 4 cases. HuCV infection peaked in August 2009, and was mostly found in children aged from 7 to 12 months. 95. 8%( 68/71 ) children aged under 24 months. ③Ten of 12 AstV infections occurred from October 2009 to January 2010, and AstV infected children were aged from 2 to 16 months. ④Of 8 AdV positive samples, there were seven serotypes: Ad31 ( 2 ), Ad2( 1 ), Ad3( 1 ), Ad5( 1 ), Ad7( 1 ), Adl2( 1 ) and Ad4l( 1 ). AdV infection peaked from January to June in 2010 and was mostly found in children aged from 7 to 12 months. ⑤Twenty-three of 300 samples were found positive to more than one viral agent, in which 12 samples contained both HRV and HuCV , and 5 samples contained both HRV and AstV, 1 sample contained both HRV and AdV, 2 samples contained both HuCV and AstV,1 sample contained both HuCV and AdV,1 sample contained both AstV and AdV, and 1 sample contained HRV, AstV and HuCV. Conclusions The results indicated clearly the impact of viral agents in causing diarrhea in children and the importance of long-term systematic surveillance. HRV was also the most important pathogen, but HuCV, AstV and AdV should be given more attention.关键词
病毒性腹泻/儿童/轮状病毒/杯状病毒/星状病毒/腺病毒/流行病学Key words
Viral diarrhea/Children/Rotavirus/Human calicivirus/Astrovirus/Adenovirus/Epidemilology引用本文复制引用
林谦,金玉,周进苏,陆芬,李晓乐,李玫,段招军..2009至2010年南京儿童医院5岁以下儿童病毒性腹泻分子流行病学特点[J].中国循证儿科杂志,2012,7(1):31-36,6.基金项目
南京市医学科技发展资金:ZKX09008 （）
江苏省卫生厅医学科技发展基金:H200949 （）
