医药导报2012，Vol.31Issue(1)：77-79,3.DOI:10.3870/yydb.2012.01.030
腺苷钴胺凝胶中腺苷钴胺的含量测定
Content Determination of Cobamamide in Cobamamide Gel
摘要
Abstract
Objective To establish a high performance liquid chromatography method for determining cobamamide in cobamamide gel. Methods A XB-C18 Uitimate column( 4. 6 mm ×250 mm , 5 μm )was used;the mobile phase was consisted of 0. 05 mol · L-1 monobasic potassium phosphate buffer solution ( adjusted to pH 3. 2 with phosphoric acid ): acetonitrile( 83 : 17 ); loading volume was 10 μL;the flow rate was 1. 0 mL · min-1 ; and the UV detection wavelength was set at 260 nm. Results The linear range for cobamamide was 10-50 |μg · mL-1 , r= 0. 999 9. The average recovery was 99.49% , with a RSD as 0. 60% ( n = 9 ). Conclusion The method is specific and accurate, which is suitable for the content determination of cobamamide in the gel.关键词
腺苷钴胺/凝胶/色谱法,高效液相/含量测定Key words
Cobamamide/Gel/HPLC/Content determination分类
医药卫生引用本文复制引用
吴燕,刘园,张福成,王明磊..腺苷钴胺凝胶中腺苷钴胺的含量测定[J].医药导报,2012,31(1):77-79,3.
