中国组织工程研究与临床康复2011，Vol.15Issue(49)：9128-9132,5.DOI:10.3969/j.issn.1673-8225.2011.49.002
不同来源骨髓间充质干细胞的体内成软骨
Chondrometaplasia of bone marrow mesenchymal stem cells of different resources in vivo
摘要
Abstract
BACKGROUND: Coagulation factor IX knockout may affect the biological characteristics of bone marrow mesenchymal stem cells (BMSCs) of the bone marrow microenvironment by affecting the hematopoietic system stability. OBJECTIVE: To characterize the biological properties of BMSCs derived from coagulation factor IX knockout mice. METHODS: BMSCs were isolated from coagulation factor EX knockout mice C57BL/6J-F9tm1Dws by using a modified adherent culture method. The cells were detected for their expression of general MSCs surface markers by using flow cytometry. The functionality was assessed for their multipotency to differentiate into osteoblasts, adipocytes and chondrocytes upon certain induction conditions.RESULTS AND DISCUSSION: The BMSCs showed spindle-shape morphology and high capacity for proliferation and self-renewal. The cells were positive for CD44, CD56, CD73, CD90, CD105, CD106, CD166and CD271, but negative for CD34 and CD11b. They demonstrated the ability to differentiate into osteoblasts, adipocytes and chondrocytes. These resufts illustrate the BMSCs derived from coagulation factor Ⅸ knockout mice C57BL / 6J-F9 tm1Dws have perfect stem cell biological characteristics. The results initially suggest that deficiency of coagulation factor IX has no effect on the biological charactaisiics of BMSCs.关键词
骨髓间充质干细胞/异种/同种异体/聚乳酸-羟基乙酸共聚物/软骨组织工程分类
基础医学引用本文复制引用
潘欣宇,崔颖,马林祥,王月田,远洋,王雪峰..不同来源骨髓间充质干细胞的体内成软骨[J].中国组织工程研究与临床康复,2011,15(49):9128-9132,5.基金项目
辽宁省自然基金资助(20062198) （20062198）
辽宁省教育厅创新团队资助(2007T110). （2007T110）
