中国组织工程研究与临床康复2011，Vol.15Issue(49)：9151-9154,4.DOI:10.3969/j.issn.1673-8225.2011.49.007
新型化学转染试剂对人脐带间充质干细胞的转染优化
Optimizing transfection of human umbilical cord-derived mesenchymal stem cells using novel chemical transfection reagents
摘要
Abstract
BACKGROUND: Genetic modification technology of human umbilical cord-derived mesenchymalstem cells (hUCMSCs) isnumerous and varies in the complexity of their efficiency, reliability and safety.OBJECTIVE: To compare transfected efficiency of different novel chemicaltransfection .eager* on hUCMSCs.METHOOS: The hUCMSCs mere transfected with Fugene HD. Lipofectamine LTX. And Attractene. Positive cells mere detectedunder fluorescent microscope, and expression efficiencies were measured by using flow cytometry. Cell viability was measuredby using trypan blue exclusion after transfection.RESULT SAND CONCLUSION: The highest transfected efficiency was observed with Lipofectamine LT×(32.S± 2.12)%<0 05). Followed by Fugene HD (4.3±0.64)%. Minimal trarsfection efficiency mas observed with Attractene (174±0.08)%.Lipofectamine LTX demonstrated the lomer cell viability (69.8iB.3y* compared mith Fugene HD (92.4±4.2)% adAttrattene(1O8.6±3.9)% (P > OSS), but the difference mas not significant. Insummary.this study demonstrates that Lipofectamine LT×is arelative ideal chemical transfection reagent for hUCMSCs.关键词
化学转染试剂/脐带间充质干细胞/基因转染/表达效率/安全性分类
基础医学引用本文复制引用
路君,赵红州,陈津,付云峰,黄梁浒,谭建明..新型化学转染试剂对人脐带间充质干细胞的转染优化[J].中国组织工程研究与临床康复,2011,15(49):9151-9154,4.基金项目
福建省自然科学基金项目(2010J05078,2010J01217). （2010J05078,2010J01217）
