中国组织工程研究与临床康复2011，Vol.15Issue(49)：9160-9164,5.DOI:10.3969/j.issn.1673-8225.2011.49.009
豚鼠脂肪间充质干细胞:一种适用于耳科实验研究的理想干细胞
Guinea pig adipose-derived mesenchymal stem cells: Ideal stem cells for experimental studies of otology
摘要
Abstract
BACKGROUND: Up to date, most of the animal experiments aboutstem cells research on the otology use the stem cells of other animal species for the guinea pig model, however, the research of guinea pig-derived stem cells is rarely reported. OBJECTIVE: To explore the differentiated potential of the guinea pig adipose mesenchymalstem cells into osteoblasis, adipocyte, nerve cells, and the application possibility for experimental study of otology.METHODS: Guinea pigs adipose tissue was isolated and cultured to the 3rd generation. Abs orbance values of cell proliferation from 1 to 10 days were detected and the immunophenotype of the cells were detected by flow cytometry. Differentiation of osteoblast. Adipocyte and nerve cells were verified.RESULTS AND CONCLUSIONS: The growth curve of adipose mesenchymal cell proliferation is similar to S-shaped.the first3 days was the incubation period after passage and the cell populations entered logarithmic growth phase from the 4hday, after the 8h day they entered into the platform period. The rejection rate of try pan blue staining was 94%-98%. Ihe total cells was ampffied by about 3600 times from primary to passage 5, The expression rate of cells of the immune phenotype CD29 was 863%, CD44 was 55.5% and CD45 was 0.4%. After differentiation of osteoblast, specific staining demonstrated the formation of mineralized nodules. After differentiation of adipocyte. Lipid droplets could be seen in cytoplasm by oil red 0 staining. After differentiation of nerve cells, cells morphology was similar to neural cells and the results of Nestn. GFAP. NF 200 staining were positive. Guinea pig adipose mes enchymal stem cells was easy to isolate, culture and amplification in vitro. They have the "stem" characteristics of mesenchymalstem cells, and they were ideal stem cells for experimental studies of otology.关键词
豚鼠/脂肪间充质干细胞/分化/耳科/神经分化分类
基础医学引用本文复制引用
郭伟,马艳,段礼府,毕晓娟,刘立中..豚鼠脂肪间充质干细胞:一种适用于耳科实验研究的理想干细胞[J].中国组织工程研究与临床康复,2011,15(49):9160-9164,5.基金项目
新疆维吾尔自治区科技厅自然科学基金(200821150). （200821150）
