重庆医学2012，Vol.41Issue(3)：214-215,219,3.DOI:10.3969/j.issn.1671-8348.2012.03.003
抗体芯片分析氯喹对巨噬细胞炎性细胞因子分泌的影响
Effects of chlorquine on inflammatory cytokines secretion from mouse macrophages by antibody chips
王艳艳1
作者信息
- 1. 成都军区总医院检验科,成都,610083
- 折叠
摘要
Abstract
Objective To investigate the effects of chlorquine on inflammatory cytokines secretion from mouse macrophages by antibody chips. Methods Proteins from the RAW 264. 7 cells in LPS group,chlorquine group,both chlorquine and LPS group,and a control group were labeled with biotin. The biotin-labeled proteins reacted with antibody chips on which the antibodies of 40 major inflammatory cytokines were prepared. The target proteins were conjugated with streptomycin antibody labeled with horseradish peroxidase(HRP) ,and signals were imaged by laser scanner. Results Compared with the LPS group,the levels of inflammatory cytokines of chlorquine group were notably decreased. Conclusion Antibody chips demonstrate chlorquine can significant inhibit inflammatory cytokines secretion from mouse macrophages indused by LPS.关键词
氯喹/内毒素类/抗体芯片/细胞因子类Key words
chlorquine/endotoxins/antibody chips/ cytokines引用本文复制引用
王艳艳..抗体芯片分析氯喹对巨噬细胞炎性细胞因子分泌的影响[J].重庆医学,2012,41(3):214-215,219,3.
