中国组织工程研究与临床康复2011，Vol.15Issue(49)：9202-9206,5.DOI:10.3969/j.issn.1673-8225.2011.49.019
氯化锂诱导毛囊干细胞定向分化中Wnt信号通路介导基因的调控
Regulation of Wnt signaling pathway involved in the differentiation of hair follicle stem cells induced by lithium chloride
摘要
Abstract
BACKGROUND: Previous studies showed that hair follicle stem cells (HFSCs) can undergo committed differentiation induced bylithium chloride (LiC I), but relating molecular regulation mechanism undisclosed.OBJECTIVE: To observe the changes of downstream molecules and target genes in Wnt signaling pathway during exogenousfactor LiCl intervenes HFSCs and to investigate the role of UCI in committed differentiation of HF SCs.METHODS: HFSCs were isolated from incised scalps of patients treated with rhytidectomy by two-steps enzyme digestionfollowed surgical microscope operation. HFSCs were subjected to antibodies-fluorescein isothiocy of cytokeratin 19 foridentification. HFSCs was intervened by exogenous factor LiCI.RESULTS AND CONCLUSION: After 5 days HFSCs treated with LiC I. HFSCs volume got larger and the nudeus/cytoplasm ratiowas reduced. In Wnt signaling pathway, the mRNA expression level of β-catenin and cyclinDI increased. GSK3β and Tcf3decreased, accompanied with c-myc increased at a lower concentration of LiCI and inversing decreased. LiCI can affecttheexpression level of β-catenin, GSK3β. Tctβ , c-myc and cyclinD1 involved in Wnt signal pathway, which may play an important rolein HFSC differentiation.关键词
氯化锂/Wnt信号通路/毛囊干细胞/β-catenin/Tcf3分类
基础医学引用本文复制引用
杨斌,向萌娟..氯化锂诱导毛囊干细胞定向分化中Wnt信号通路介导基因的调控[J].中国组织工程研究与临床康复,2011,15(49):9202-9206,5.基金项目
国家自然科学基金项目(30772099) （30772099）
北京自然科学基金项目(7112111). （7112111）
