中国组织工程研究与临床康复2011，Vol.15Issue(49)：9211-9214,4.DOI:10.3969/j.issn.1673-8225.2011.49.021
重组反转录病毒碱性成纤维细胞生长因子基因转染对人骨髓基质细胞成骨的影响
Effect of recombinant retrovirus pLXSN/basic fibroblast growth factor transfection on osteogenesis of human marrow stroma cells
周荣兴 1陈华 2郭军 1吕慧成 1杨铁翼1
作者信息
- 1. 内蒙古医学院第二附属医院创伤骨科,内蒙古自治区呼和浩特市,010000
- 2. 内蒙古监狱管理局第一医院内科,内蒙古自治区呼和浩特市,010000
- 折叠
摘要
Abstract
BACKGROUND: In home and abroad have many studies on basic fibroblast growth factor (Bfgf) transfedion for improving thegrowth of muscle and vessel, but little studies on Bfgf for improving the growth of osteogenesis.OBJECTIVE: To investigate the effect of recombinant retrovirus Plxsn/Bfgf transfedion on osteogenic potential of humanmarrow stroma cells (hMSCs).METHODS: hMSCs isolated from health volunteers, cultured and purified in vitro were randomly divided into four groups.Retrovirus Plxsn/Bfgf group with recombinant retrovirus Plxsn/Bfgf was added into culture medium. Retrovirus Plxsngroup with recombinant retrovirus Plxsn was added to take the place of retrovirus Plxsn/Bfgf. Positivecontrol group withdexamethasone, glycerophosphate and ascorbic were added into culture medium. Blank control group without any specialtreatment.RESULTS AND CONCLUSION: hMSCs were uniform spindle-shape fibroMast after several passages. After treatment hMSCsin retrovirus Plxsn/Bfgf and positive control groups graduaty showed flatshape. Immunohistochemical staining showed thatthe level of Bfgf expression in retrovirus Plxsn/Bfgf group was higher than that of the other three groups. Mineralizednodules and bone collagen were formed, and alkcaine phosphatase activity was improved in retrovirus Plxsnafgf and positivecontrol groups. Recombinant of retrovirus Plxsn/Bfgf gene transfedion into hMSCs can promote the osteogenic potential ofhMSCs.关键词
成纤维细胞生长因子/骨髓基质细胞/反转录病毒/基因转染/骨生成分类
基础医学引用本文复制引用
周荣兴,陈华,郭军,吕慧成,杨铁翼..重组反转录病毒碱性成纤维细胞生长因子基因转染对人骨髓基质细胞成骨的影响[J].中国组织工程研究与临床康复,2011,15(49):9211-9214,4.
